Spectrophotometer are the common instruments to be used in chemistry. The spectrophotometry is a quantitative calculation of the transmission or reflection qualities of a material as the function of the wavelength. It is more specific than the common term electromagnetic spectroscopy in a way that it is concerned with the visible light, near infrared and near ultraviolet, however, doesn’t concern with time resolved spectroscopic methods. The spectroscopy utilizes the photometers that are able to measure the intensity of a light beam as a function of its wavelength (color) called spectrometers.

A spectrometer is a special measuring tool for measuring transmission or reflection of solutions, opaque or transparent solids like polished glass or various gases.  These are also designed for measuring the diffusivity of the light ranges that come under almost 200 nm to 2500 nm, using various calibrations and controls. In these light ranges, the calibrations are required on a machine using standards that differ in type on the basis of wavelength of photometric determination. As far as the important features of spectrophotometers are concerned, these include spectral bandwidth, which is the range of colors it is able to transmit by test sample, the proportion of sample transmission, the logarithmic range of sample absorption and often a percentage of the reflective measurement.

Spectrophotometers are usually used in the experiments where the equilibrium constant of the solution is to be determined. In addition, the use of these instruments is also seen in various fields like material science, physics, molecular biology, chemistry and bio chemistry. These are also heavily used in various industries like laser, semiconductors, manufacturing, optical, forensic examination, printing and laboratories as well for the analysis of chemical substances. Lastly, these are also applied to measure the presence of substances in a target, based on the calibration or control, also let you measure how much the presence is by measuring observed wavelengths.

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